MutS2 Promotes Homologous Recombination in Bacillus subtilis
نویسندگان
چکیده
منابع مشابه
Genetic Recombination in Bacillus subtilis 168: Effect of DhelD on DNA Repair and Homologous Recombination
The B. subtilis DhelD allele rendered cells proficient in transformational recombination and moderately sensitive to methyl methanesulfonate when present in an otherwise Rec strain. The DhelD allele was introduced into rec-deficient strains representative of the a (recF strain), b (addA addB), g (recH), « (DrecU), and z (DrecS) epistatic groups. The DhelD mutation increased the sensitivity to D...
متن کاملGenetic recombination in Bacillus subtilis 168: effect of DeltahelD on DNA repair and homologous recombination.
The B. subtilis DeltahelD allele rendered cells proficient in transformational recombination and moderately sensitive to methyl methanesulfonate when present in an otherwise Rec(+) strain. The DeltahelD allele was introduced into rec-deficient strains representative of the alpha (recF strain), beta (addA addB), gamma (recH), epsilon (DeltarecU), and zeta (DeltarecS) epistatic groups. The Deltah...
متن کاملGenetic recombination in Bacillus subtilis 168: effects of recU and recS mutations on DNA repair and homologous recombination.
Bacillus subtilis recombination-deficient mutants were constructed by inserting a selectable marker (cat gene) into the yppB and ypbC coding regions. The yppB:cat and ypbC:cat null alleles rendered cells sensitive to DNA-damaging agents, impaired plasmid transformation (25- and 100-fold), and moderately affected chromosomal transformation when present in an otherwise Rec+ B. subtilis strain. Th...
متن کاملRecombination between compatible plasmids containing homologous segments requires the Bacillus subtilis recE gene product.
Plasmid pSL103 was previously constructed by cloning a Trp fragment (approximately 2.3 X 10(6) daltons) from restriction endonuclease EcoRI-digested chromosome DNA of Bacillus pumilus using the neomycin-resistance plasmid pUB110 (approximately 2.8 X 10(6) daltons) as vector and B. subtilis as transformation recipient. In the present study the EcoRI Trp fragment from pSL103 was transferred in vi...
متن کاملUSP4 Auto-Deubiquitylation Promotes Homologous Recombination.
Repair of DNA double-strand breaks is crucial for maintaining genome integrity and is governed by post-translational modifications such as protein ubiquitylation. Here, we establish that the deubiquitylating enzyme USP4 promotes DNA-end resection and DNA repair by homologous recombination. We also report that USP4 interacts with CtIP and the MRE11-RAD50-NBS1 (MRN) complex and is required for Ct...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2017
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.00682-16